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adeno-associated virus (aav) encoding gcamp6s  (Addgene inc)


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    Addgene inc adeno-associated virus (aav) encoding gcamp6s
    Adeno Associated Virus (Aav) Encoding Gcamp6s, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adeno-associated virus (aav) encoding gcamp6s/product/Addgene inc
    Average 90 stars, based on 1 article reviews
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    (A) Schematic illustration of stereotaxic injections of retrograde microspheres to label L5 extratelencephalic neurons (ETs, green), and viral vectors (AAVs) for expression of ChR2 in thalamocortical (TC) inputs and tdTomato in L6 corticothalamic <t>neurons</t> <t>(CTs,</t> red) in Ntsr1-Cre mice. (B) Schematic illustration of slice electrophysiology experiment involving photostimulation of ChR2 expressing TC afferents and simultaneous (dual) recording from an ET (green) and a CT (red). (C) Images in 4X magnification showing the extent of <t>ACtx</t> area in bright-field (left), green-labeled ETs and TC axons (middle) and red-labeled CTs. (D) Average CT/ET EPSC ratio after optogenetically stimulating thalamic L5-6 input 1d and 7d after SE and NE. (1d SE: 18 cells/6 mice; 1d NE: 26 cells/9 mice; 7d SE: 10 cells/6 mice; 7d NE: 10 cells/6 mice). Asterisks indicate significant differences (***p<0.001, two-way ANOVA and Bonferroni correction for multiple comparisons). (E, F) Representative traces of EPSCs in dual recordings from both CT (solid line) and ET (dotted line) neurons evoked by maximal photostimulation of L5-6 thalamocortical inputs in 1d SE (E, left) and 1d NE (E, right); 7d SE (F, left) and 7d NE (F, right). Different colored traces represent different pairs of simultaneously recorded CTs and ETs. Detailed statistical values are listed in .
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    (A) Schematic illustration of stereotaxic injections of retrograde microspheres to label L5 extratelencephalic neurons (ETs, green), and viral vectors (AAVs) for expression of ChR2 in thalamocortical (TC) inputs and tdTomato in L6 corticothalamic <t>neurons</t> <t>(CTs,</t> red) in Ntsr1-Cre mice. (B) Schematic illustration of slice electrophysiology experiment involving photostimulation of ChR2 expressing TC afferents and simultaneous (dual) recording from an ET (green) and a CT (red). (C) Images in 4X magnification showing the extent of <t>ACtx</t> area in bright-field (left), green-labeled ETs and TC axons (middle) and red-labeled CTs. (D) Average CT/ET EPSC ratio after optogenetically stimulating thalamic L5-6 input 1d and 7d after SE and NE. (1d SE: 18 cells/6 mice; 1d NE: 26 cells/9 mice; 7d SE: 10 cells/6 mice; 7d NE: 10 cells/6 mice). Asterisks indicate significant differences (***p<0.001, two-way ANOVA and Bonferroni correction for multiple comparisons). (E, F) Representative traces of EPSCs in dual recordings from both CT (solid line) and ET (dotted line) neurons evoked by maximal photostimulation of L5-6 thalamocortical inputs in 1d SE (E, left) and 1d NE (E, right); 7d SE (F, left) and 7d NE (F, right). Different colored traces represent different pairs of simultaneously recorded CTs and ETs. Detailed statistical values are listed in .
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    Image Search Results


    (A) Schematic illustration of stereotaxic injections of retrograde microspheres to label L5 extratelencephalic neurons (ETs, green), and viral vectors (AAVs) for expression of ChR2 in thalamocortical (TC) inputs and tdTomato in L6 corticothalamic neurons (CTs, red) in Ntsr1-Cre mice. (B) Schematic illustration of slice electrophysiology experiment involving photostimulation of ChR2 expressing TC afferents and simultaneous (dual) recording from an ET (green) and a CT (red). (C) Images in 4X magnification showing the extent of ACtx area in bright-field (left), green-labeled ETs and TC axons (middle) and red-labeled CTs. (D) Average CT/ET EPSC ratio after optogenetically stimulating thalamic L5-6 input 1d and 7d after SE and NE. (1d SE: 18 cells/6 mice; 1d NE: 26 cells/9 mice; 7d SE: 10 cells/6 mice; 7d NE: 10 cells/6 mice). Asterisks indicate significant differences (***p<0.001, two-way ANOVA and Bonferroni correction for multiple comparisons). (E, F) Representative traces of EPSCs in dual recordings from both CT (solid line) and ET (dotted line) neurons evoked by maximal photostimulation of L5-6 thalamocortical inputs in 1d SE (E, left) and 1d NE (E, right); 7d SE (F, left) and 7d NE (F, right). Different colored traces represent different pairs of simultaneously recorded CTs and ETs. Detailed statistical values are listed in .

    Journal: bioRxiv

    Article Title: Cell-type-specific plasticity in synaptic, intrinsic, and sound response properties of deep-layer auditory cortical neurons after noise trauma

    doi: 10.1101/2025.07.09.663954

    Figure Lengend Snippet: (A) Schematic illustration of stereotaxic injections of retrograde microspheres to label L5 extratelencephalic neurons (ETs, green), and viral vectors (AAVs) for expression of ChR2 in thalamocortical (TC) inputs and tdTomato in L6 corticothalamic neurons (CTs, red) in Ntsr1-Cre mice. (B) Schematic illustration of slice electrophysiology experiment involving photostimulation of ChR2 expressing TC afferents and simultaneous (dual) recording from an ET (green) and a CT (red). (C) Images in 4X magnification showing the extent of ACtx area in bright-field (left), green-labeled ETs and TC axons (middle) and red-labeled CTs. (D) Average CT/ET EPSC ratio after optogenetically stimulating thalamic L5-6 input 1d and 7d after SE and NE. (1d SE: 18 cells/6 mice; 1d NE: 26 cells/9 mice; 7d SE: 10 cells/6 mice; 7d NE: 10 cells/6 mice). Asterisks indicate significant differences (***p<0.001, two-way ANOVA and Bonferroni correction for multiple comparisons). (E, F) Representative traces of EPSCs in dual recordings from both CT (solid line) and ET (dotted line) neurons evoked by maximal photostimulation of L5-6 thalamocortical inputs in 1d SE (E, left) and 1d NE (E, right); 7d SE (F, left) and 7d NE (F, right). Different colored traces represent different pairs of simultaneously recorded CTs and ETs. Detailed statistical values are listed in .

    Article Snippet: CTs were transduced with GCaMP6s through intracranial injection into the ACtx (AAV5-Syn-FLEX-GCaMP6s-WPRE-SV40, titer: 7e 12 gc/mL, Addgene) or through transgenic crossing with the Ai148 mouse line.

    Techniques: Expressing, Labeling